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Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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Biotium biotium cell viability cytotoxicity kit
Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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Image Search Results


Cell-scaffold interaction (A) Live/Dead staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Journal: iScience

Article Title: Ready-to-use 3D bioprinted scaffolds from natural materials loaded with patient’s PRGF for personalized skin regeneration

doi: 10.1016/j.isci.2026.116185

Figure Lengend Snippet: Cell-scaffold interaction (A) Live/Dead staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Article Snippet: LIVE/DEAD® viability/cytotoxicity kit , Fisher Scientific , Cat# L3224.

Techniques: Staining, Imaging